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Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-myc3-p27
Plasmid#19937DepositorInsertCDKN1B cyclin-dependent kinase inhibitor 1B (CDKN1B Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-mTrim21ΔPRYSPRY
Plasmid#105518PurposeExpression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of PRYSPRY domain (aa1-281)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-GFP-SF3B6-FLAG
Plasmid#86870PurposeTo express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.DepositorInsertSF3B6 (SF3B6 Human)
UseTagsEGFP and FLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKH159_FLAG-APEX2-YIPF4 WT
Plasmid#214978PurposeFlag-APEX2 tagged YIPF4DepositorInsertFLAG-APEX2-YIPF4 (YIPF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH161_FLAG-APEX2-YIPF3 WT
Plasmid#214980PurposeFlag-APEX2 tagged YIPF3DepositorInsertFLAG-APEX2-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH143_EGFP-YIPF3 LIR mut
Plasmid#214975PurposeGFP tagged LIR mutant YIPF3DepositorInsertGFP-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH142_EGFP-YIPF3 WT
Plasmid#214974PurposeGFP tagged YIPF3DepositorInsertGFP-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FNIP1
Plasmid#72292PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-EGFP-HaloTag
Plasmid#169451Purposeexpression of dCas9-EGFP-HaloTagDepositorInsertdCas9-EGFP-HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRA108
Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal80-3xP3-RFP
Plasmid#127562PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1- Ncad-GFP
Plasmid#35676DepositorInsertNcad (cdh2 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK364.306
Plasmid#192642PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-hfYFP
Plasmid#191201PurposeConstitutive CAG-driven expression of hfYFP in animals using AAVDepositorInsertHyperfolder YFP
UseAAVTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCAGAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only