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Addgene

pcDNA3-myc3-p27
(Plasmid #19937)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19937 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3-myc3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5486
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDKN1B cyclin-dependent kinase inhibitor 1B
  • Alt name
    p27
  • Alt name
    Kip1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    597
  • GenBank ID
    ENST00000228872
  • Entrez Gene
    CDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Data not available (not destroyed)
  • 3′ cloning site Data not available (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-p27 was a gift from Yue Xiong (Addgene plasmid # 19937 ; http://n2t.net/addgene:19937 ; RRID:Addgene_19937)
  • For your References section:

    Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Zariwala M, Liu J, Xiong Y. Oncogene. 1998 Nov 26. 17(21):2787-98. 10.1038/sj.onc.1202505 PubMed 9840943