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Showing: 4181 - 4200 of 5448 results

  3. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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  4. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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  12. pHLmMBP-6

    Plasmid
    #72346
    Purpose
    Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72346-plasmid-map-sequence-id-133754

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  13. nCas9-PPE

    Plasmid
    #140445
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140445-plasmid-map-sequence-id-296171

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  16. pHLmMBP-2

    Plasmid
    #72344
    Purpose
    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72344-plasmid-map-sequence-id-132067

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  20. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197101-plasmid-map-sequence-id-390718

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Showing: 4181 - 4200 of 5448 results