Addgene

We narrowed to 28,572 results for: uros

Showing: 10451 - 10500 of 28572 results

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pGWB504

Plasmid

#74846

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB412

Plasmid

#74806

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 miR-200c-141

Plasmid

#81020

Purpose

Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

Depositor

Thomas Westbrook, Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

miR-200c (MIR200C Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rubicon

Plasmid

#21636

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Rubicon (1700021K19Rik Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

*(see below)

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha

Plasmid

#35555

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Use

Tags

Flag and His

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ10704

Plasmid

#183957

Purpose

U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

SKSKSO array

Use

Lentiviral

Tags

EF1a-Puro-T2A-TagBFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 human, Human, Synthetic)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.CMV.flex.hM3Dq.Cherry

Plasmid

#170360

Purpose

Cre-inducible lentiviral vector expressing hM3Dq

Depositor

Johan Jakobsson

Article

Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

Insert

flex.hM3Dq.Cherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Relb

Plasmid

#139810

Purpose

doxycycline-inducible expression of mouse Relb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Relb (Relb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB541

Plasmid

#74875

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB542

Plasmid

#74876

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2-DEST(f)

Plasmid

#190226

Purpose

Gateway destination vector, empty AAV vector backbone

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC-nSyb-IVS-PhiC31

Plasmid

#133868

Purpose

Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

Plasmid

#166611

Purpose

Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB413

Plasmid

#74807

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB433

Plasmid

#74824

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-tetO-Stat3

Plasmid

#139805

Purpose

doxycycline-inducible expression of mouse Stat3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB553

Plasmid

#74883

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB415

Plasmid

#74809

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8e-dimer

Plasmid

#138490

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB510

Plasmid

#74852

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
slc1a3b:myrGFP-P2A-H2AmCherry

Plasmid

#170208

Purpose

Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGFP-P2A-H2AmCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn1

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.FRB-3myc-KAP3B

Plasmid

#193939

Purpose

Mammalian expression of KAP 3B

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

KAP 3B (KIFAP3 Human)

Use

Tags

FRB

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NG-ABE8-dimer

Plasmid

#138497

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HiBiT-CD81

Plasmid

#162593

Purpose

N-terminally HiBiT-tagged human CD81 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD81 (CD81 human, Human, Synthetic)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

Plasmid

#183804

Purpose

AAV vector to express nuclear localized eGFP from a Gad65 promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eGFP-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB535

Plasmid

#74873

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PBCAG-EGFR

Plasmid

#40974

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

EGFR (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP104-melanopsin-mCherry

Plasmid

#122630

Purpose

AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

Insert

melanopsin-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

GFAP104

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB515

Plasmid

#74857

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CT3G-CERP2-MG-IRES2-mNeonGreen

Plasmid

#175508

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G-mCMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Use

Tags

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Stat5

Plasmid

#139832

Purpose

doxycycline-inducible expression of mouse Stat5 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat5 (Stat5a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Tags

Expression

Mutation

Promoter

U6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-DIO-dAPEX2

Plasmid

#117174

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry N1 TOMM22

Plasmid

#157762

Purpose

Expression of TOMM22:mcherry in cells

Depositor

Marc Tramier

Article

Bertolin et al Elife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

TOMM22 (TOMM22 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB521

Plasmid

#74863

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GG-EBNA-OSK2M2L1-PP

Plasmid

#102902

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OSK2M2L1-gRNAs-PGK-puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Use

Tags

VSV and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB16 (pmyo-3::QuasAr)

Plasmid

#130272

Purpose

Expresses the voltage sensor QuasAr in BWMs of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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