PBCAG-EGFR
(Plasmid #40974)

• Depositing Lab: Joseph Loturco
• Publication: Chen et al J Neurosci Methods. 2012 Jun 15;207(2):172-80. Epub 2012 Apr 11.

Backbone

• Vector backbone: PBCAG-eGFP
• Backbone size w/o insert (bp): 3633
• Total vector size (bp): 9200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFR
• Alt name: Epidermal growth factor receptor
• Species: H. sapiens (human)
• Insert Size (bp): 3633
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Matthew Meyerson
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry