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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-cys-His6-OtUBD

Plasmid

#190091

Purpose

Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pW5Y_Apr

Plasmid

#158211

Purpose

BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q842gp160

Plasmid

#100931

Purpose

Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6A-EGFR ECD (1-644)

Plasmid

#42666

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ECD (1-644) (EGFR Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGWB445

Plasmid

#74831

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mCherry

Plasmid

#62632

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Plasmid

#209296

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-6

Plasmid

#50756

Purpose

Expresses MagFRET-6 in the cytosol of mammalian cells. Weak affinity control FRET sensor for MagFRET-1.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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p426MET25_sfpHluorin (MRV55)

Plasmid

#115697

Purpose

superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

Depositor

Eckhard Boles

Article

Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

Insert

sfpHluorin

Use

Tags

Expression

Yeast

Mutation

for introduced mutations see publication

Promoter

MET25

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

Plasmid

#192193

Purpose

Lenti-BCMA-CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBOB-VE-cadherin-tdTomato

Plasmid

#118733

Purpose

To express VE-cadherin tagged with tdTomato on C-terminus

Depositor

John Cooper

Insert

Cadherin 5 (CD144) (CDH5 Human)

Use

Lentiviral

Tags

tdTomato

Expression

Mutation

I503T (Common SNP-see comments)

Promoter

CMV

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δβ-catenin-pcDNA3

Plasmid

#45772

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δβ-catenin (CDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Ends at amino acid 844. Last 35 amino acids dele…

Promoter

CMV

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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WT Smarca4-sfGFP

Plasmid

#107056

Purpose

Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

Depositor

Courtney Hodges, Jerry Crabtree

Article

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

Super-folder GFP (sfGFP)

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-iLACCO1.2

Plasmid

#208101

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d/LacZ

Plasmid

#66201

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

AtU3d

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-EPSIN 1

Plasmid

#22228

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

please see depositor comment below

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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