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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRESneo2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-alpha Tubulin
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Alt nameEGFP-Tubulin
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Alt namek-alpha-1
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SpeciesH. sapiens (human)
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Insert Size (bp)2100
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Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRESneo-EGFP-alpha Tubulin was a gift from Patricia Wadsworth (Addgene plasmid # 12298 ; http://n2t.net/addgene:12298 ; RRID:Addgene_12298) -
For your References section:
Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Rusan NM, Fagerstrom CJ, Yvon AM, Wadsworth P. Mol Biol Cell. 2001 Apr . 12(4):971-80. 10.1091/mbc.12.4.971 PubMed 11294900