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Addgene

pIRESneo-EGFP-alpha Tubulin
(Plasmid #12298)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12298 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRESneo2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-alpha Tubulin
  • Alt name
    EGFP-Tubulin
  • Alt name
    k-alpha-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESneo-EGFP-alpha Tubulin was a gift from Patricia Wadsworth (Addgene plasmid # 12298 ; http://n2t.net/addgene:12298 ; RRID:Addgene_12298)
  • For your References section:

    Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Rusan NM, Fagerstrom CJ, Yvon AM, Wadsworth P. Mol Biol Cell. 2001 Apr . 12(4):971-80. 10.1091/mbc.12.4.971 PubMed 11294900