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Addgene

pCAG-mGFP-Actin
(Plasmid #21948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 5802
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    betaActin
  • Alt name
    ACTB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1128
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gaccccaacgagaagcgcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mGFP-Actin was a gift from Ryohei Yasuda (Addgene plasmid # 21948 ; http://n2t.net/addgene:21948 ; RRID:Addgene_21948)

  • For your References section:

    Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP. Murakoshi H, Lee SJ, Yasuda R. Brain Cell Biol. 2008 Aug . 36(1-4):31-42. 10.1007/s11068-008-9024-9 PubMed 18512154
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