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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5150
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH-Ras G12V Y40C
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Alt nameHRas
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SpeciesH. sapiens (human)
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MutationContains G12V activating mutation. The Y40C mutation allows binding and activation of PI3K, but not of Raf or Ral-GEF.
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Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySee Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe puro H-Ras G12V Y40C was a gift from Bob Weinberg (Addgene plasmid # 12276 ; http://n2t.net/addgene:12276 ; RRID:Addgene_12276) -
For your References section:
Species- and cell type-specific requirements for cellular transformation. Rangarajan A, Hong SJ, Gifford A, Weinberg RA. Cancer Cell. 2004 Aug . 6(2):171-83. 10.1016/j.ccr.2004.07.009 PubMed 15324700