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Plasmid#49202PurposeFluorescent reporter for cGMPDepositorInsertFlincG3
UseTagsHis tag + other tagsExpressionBacterial, Insect, and Mamm…MutationMet to Lys in cpEGFP region similar to CGaMP3PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB411
Plasmid#74805PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB435
Plasmid#74825PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-GBA-N370S-tmpknot-epegRNA
Plasmid#214091PurposeLentiviral vector expressing epegRNA to induce GBA N370S mutationDepositorInsertGBA N370S epegRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9
Plasmid#89995PurposeExpresses high levels of WT Cas9 for genome editingDepositorInserthCas9
UseTags3xFlag, SV40 NLS, and T2A peptideExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-tTA
Plasmid#99119PurposeAn AAV genome that expresses tTA from the hSyn1 promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterhSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mRuby3-WPRE
Plasmid#193343PurposeAAV-mediated expression of mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform1
Plasmid#70173PurposeMammalian expression of hsOPA1 isoform 1DepositorInserthsOPA1 isoform 1 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen-WPRE
Plasmid#193341PurposeAAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tTA
Plasmid#193338PurposeAAV-mediated expression of tTA under the CAG promoter in mammalian cellsDepositorInserttTA2
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1(pCMV5)
Plasmid#12148DepositorInsertFoxo1 (Foxo1 Mouse)
UseTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE LOX Cloning template vector
Plasmid#139651PurposeCloning template for ORANGE-CAKE conditional knock-in constructsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mNeonGreen
Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGWB445
Plasmid#74831PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA
Plasmid#129708PurposeExpress cre-dependent KASH-EGFP and scramble shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianMutationPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-BAP1
Plasmid#22539DepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-2A-TetanusToxin
Plasmid#166603PurposeEncodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TREDepositorInsertEGFP-2A-Tetanus Toxin
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB417
Plasmid#74811PurposeGateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-GCaMP6f-P2A-H2B-PAmCherry
Plasmid#133419PurposeFluorescent reporter for calcium imaging and photoactivationDepositorInsertGCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-FLPo
Plasmid#118027PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-DIO-FLPo
UseAAVTagsExpressionMutationPromoterTREAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-EGFP-TM-IRES-Pac
Plasmid#183604PurposeMammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHuman PDGFRB transmembrane domain and Mouse IgGK …ExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP HA-Parkin
Plasmid#38248DepositorInsertParkin (PRKN Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSlug-4
Plasmid#40648DepositorInsertsh-mSlug-4 (Snai2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-GluR2(Q)
Plasmid#24002DepositorInsertGluR2 (Gria2 Rat)
UseTagsGluR2 signal peptide (aa1-24) and SEP (supereclip…ExpressionMammalianMutationR607QPromoterCMVAvailable sinceApril 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-CYLD
Plasmid#22544DepositorInsertCYLD (CYLD Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX317 YAP1
Plasmid#193683PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiRhoA2G
Plasmid#40179DepositorInsertRhoA Biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-FLAG-KD LKB1
Plasmid#8593DepositorInsertLKB1 (STK11 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationK78I: lysine 78 mutated to isoleucine; kinase deadPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBa.PEX3-tdTomato-FKBP
Plasmid#193941PurposeMammalian expression of tdTomato-tagged PEX3DepositorInsertPEX3 (PEX3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGCaMP6s-P2A-H2AmCherry
Plasmid#170209PurposeDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleusDepositorInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGCaMP6s-P2A-H2AmCherryExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e
Plasmid#138492PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (CP1028)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tTA
Plasmid#99118PurposeAn AAV genome that ubiquitously expresses tTA from the CAG promoterDepositorHas ServiceAAV2InserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSlug-3
Plasmid#40647DepositorInsertsh-mSlug-3 (Snai2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mNeonGreen
Plasmid#99135PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoterDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSOX9-5
Plasmid#40646DepositorInsertsh-mSOX9-5 (Sox9 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGWB544
Plasmid#74878PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HiBiT-CD63
Plasmid#162591PurposeN-terminally HiBiT-tagged human CD63 under CAG promoterDepositorInserthuman CD63 (CD63 human, Human, Synthetic)
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB518
Plasmid#74860PurposeGateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
Plasmid#32520DepositorInsertConstitutively active Rheb (S16H) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationS16H to make constitutively activePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWB408
Plasmid#74802PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-EGFP-WPRE-pA
Plasmid#37085PurposeExpresses EGFP in cells lacking Cre (Cre-Off)DepositorInsertEGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-mCherry
Plasmid#193060Purposedoxycycline-inducible expression of mCherry in mammalian cellDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB513
Plasmid#74855PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only