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PurposeAAV vector for sparse and bright labeling in a cell-type specific manner
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTRE-DIO-FLPo
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SpeciesSynthetic
- Promoter TRE
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-TRE-DIO-FLPo was a gift from Minmin Luo (Addgene plasmid # 118027 ; http://n2t.net/addgene:118027 ; RRID:Addgene_118027) -
For your References section:
Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M. Nat Methods. 2018 Nov 19. pii: 10.1038/s41592-018-0184-y. doi: 10.1038/s41592-018-0184-y. 10.1038/s41592-018-0184-y PubMed 30455464