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Plasmid#191550PurposeExpression of spCas9 and sgRNA targetting ZER1DepositorInsertspCas9 (ZER1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpAGS-dGoLoco1-GFP
Plasmid#185577Purposefor overexpressionDepositorInsertAGS-dGoLoco1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationdeletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2Aq(2nd)
Plasmid#183089Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-TalO8 (Blank-CoTrIP)
Plasmid#178303PurposeBlank CoTrIP plasmidDepositorTypeEmpty backboneUseTagsNAExpressionYeastMutationPromoterNAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK88
Plasmid#176612PurposeTo manipulate ligand spacing.DepositorInsertDNA-CARy- aTM (aa291-404)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup_771-1132_C
Plasmid#146030PurposeInsect Expression of DmCup_771-1132DepositorInsertDmCup_771-1132 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_771-1132_C
Plasmid#146034PurposeInsect Expression of DmCup_771-1132DepositorInsertDmCup_771-1132 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C
Plasmid#146035PurposeInsect Expression of DmCup-Y342AL347ADepositorInsertDmCup-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSA293-CHEQ-seq
Plasmid#174669PurposeReporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.DepositorTypeEmpty backboneUseEnhancer reporterTagsExpressionMutationPromoterSCP1Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH805_pJH_LldR_Pcon-sfGFP.0TAG
Plasmid#163736PurposeConstitutive expression of green fluorescent protein encoding zero stop codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-ORF68 (tagless)
Plasmid#166025PurposeExpresses ORF68 in mammalian cellsDepositorInsertORF68
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-EnRRbpj
Plasmid#160925Purposedominant-negative construct to inhibit Notch signalingDepositorInsertEnRRbpj
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8208 MSCV-Neo C-HA EMPTY
Plasmid#163310PurposeEmpty vector controlDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-Rictor-shRNA
Plasmid#157930PurposeKnockdown of RictorDepositorInsertRictor shRNA
UseLentiviral and RNAiTagstdTomatoExpressionMutationPromotermouse U6Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-A1
Plasmid#114149PurposeExpresses ClbH-A1DepositorInsertclbH-A1
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationContains only the A1 domain of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pre-Ubc4 BrG pUC19
Plasmid#111322Purposefor transcription to Ubc4 pre-mRNADepositorInsertUBC4 (UBC4 Budding Yeast)
UseTagsExpressionBacterialMutationintron has branch-site A to G mutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 dATG1 (1188)
Plasmid#66918Purposebacterial expression of dATG1DepositorInsertATG1
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP757-1
Plasmid#99493Purpose6XHis::TEV::3XFlag::linker::MycDepositorInsert6XHis::TEV::3XFlag::linker::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP605-1
Plasmid#99482PurposeMyc::TEV::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::TEV::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only