Skip to main content
Addgene
Showing: 8281 - 8320 of 148587 results

  3. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

    Enlarge

  4. pCMV-AAV5cap

    Plasmid
    #198015
    Purpose
    For high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM library
    Depositor
    George Church
    Article
    Jain et al bioRxiv 2023.01.31.526541
    Insert
    AAV5 cap
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    198015-plasmid-map-sequence-id-390024

    Enlarge

  5. pDAC446

    Plasmid
    #195239
    Purpose
    Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195239-plasmid-map-sequence-id-382947

    Enlarge

  6. pSG5 PPAR alpha

    Plasmid
    #22751
    Depositor
    Bruce Spiegelman
    Insert
    PPARalpha (Ppara Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    22751-plasmid-map-sequence-id-160481

    Enlarge

  9. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Tags
    Expression
    Mutation
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

    Enlarge

  10. DDX3X in pENTR

    Plasmid
    #216572
    Purpose
    gateway cloning donor vector containing DDX3X
    Depositor
    Christopher Loewen, Douglas Allan
    Insert
    DDX3X (DDX3X Human)
    Use
    Gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216572-plasmid-map-sequence-id-426667

    Enlarge

  11. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid
    #206042
    Purpose
    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
    Depositor
    Alisa Piekny
    Article
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    Use
    CRISPR and Synthetic Biology; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206042-plasmid-map-sequence-id-411117

    Enlarge

  15. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

    Enlarge

  19. pCS2+ H2B-mTagBFP2

    Plasmid
    #99267
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mTagBFP2-3XHA
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99267-plasmid-map-sequence-id-192954

    Enlarge

  22. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-137253

    Enlarge

  28. pcDNA3.1-GGamma12-GFP2

    Plasmid
    #166781
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma12-GFP2 (GNG12 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166781-plasmid-map-sequence-id-331013

    Enlarge

  34. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

    Enlarge

  39. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid
    #178335
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV CAG iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    178335-plasmid-map-sequence-id-350412

    Enlarge

Showing: 8281 - 8320 of 148587 results