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Addgene

pLVX-CMV-Galpha-oA ONE-GO
(Plasmid #189732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-IRES-Hygro
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Galpha-oA ONE-GO
  • Species
    R. norvegicus (rat)
  • Mutation
    I250 (silent mutation in YFP)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    GNAO
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-CMV-Galpha-oA ONE-GO was a gift from Mikel Garcia-Marcos (Addgene plasmid # 189732 ; http://n2t.net/addgene:189732 ; RRID:Addgene_189732)

  • For your References section:

    Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M. Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028. 10.1016/j.cell.2024.01.028 PubMed 38412860
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