pLVX-CMV-Galpha-12 ONE-GO
(Plasmid
#189739)
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PurposeGPCR/G protein BRET biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-IRES-Hygro
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGalpha-12 ONE-GO
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SpeciesH. sapiens (human)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
Gene/Insert 2
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Gene/Insert nameGNA12
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SpeciesH. sapiens (human)
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Entrez GeneGNA12 (a.k.a. HG1M1, NNX3, RMP, gep)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-CMV-Galpha-12 ONE-GO was a gift from Mikel Garcia-Marcos (Addgene plasmid # 189739 ; http://n2t.net/addgene:189739 ; RRID:Addgene_189739) -
For your References section:
Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M. Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028. 10.1016/j.cell.2024.01.028 PubMed 38412860