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NCKX4 scFv [N414/25]

Plasmid

#190553

Purpose

Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-Pde4a

Plasmid

#105463

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde4a (Pde4a Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Flpo Flp-in replacement vector

Plasmid

#61572

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Flpo

Use

Recombinase-mediated cassette exchange using flp …

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pNrl-Cre

Plasmid

#13780

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Nrl promoter-Cre (Nrl Mouse)

Use

Cre/Lox

Tags

Cre

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV MEKK3 PB1

Plasmid

#12185

Depositor

Gary Johnson

Article

Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

Insert

MEKK3 (Map3k3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

PB1 domain: aa 36-127

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-SNAP

Plasmid

#208773

Purpose

To express C2m2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Use

Tags

SNAP-tag

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-mKate

Plasmid

#208765

Purpose

To express C2m2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-mKate (Mfge8 Mouse)

Use

Tags

mKate

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mPCSK9)

Plasmid

#170122

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMiniT:SEP-GluN2A TKIT donor

Plasmid

#169429

Purpose

Donor DNA for TKIT knockin of SEP to NR2A in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgApc/Cre

Plasmid

#89641

Purpose

Expresses an Apc-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Apc (Apc Mouse)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Dlx5

Plasmid

#15538

Depositor

John Rubenstein

Article

Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

Insert

Dlx5 (Dlx5 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Cntn2-Fc-His

Plasmid

#72066

Purpose

Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn2 (Cntn2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2N620K

Plasmid

#22631

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Aug 31. ():.

Insert

Ror2 N620K (Ror2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed Asparagine 620 to Lysine

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13

Plasmid

#61087

Purpose

Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13, excluding exon 7, 11, 14-17

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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