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pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Tags

Expression

Mutation

Promoter

human SYN1

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

Plasmid

#63884

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α-loxP-GCaMP6f-loxP-WPRE

Plasmid

#233288

Purpose

AAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.

Depositor

Dayu Lin

Article

Fang et al Neuron. 2018 Apr 4;98(1):192-207.e10. do

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

EF1α

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

Plasmid

#186049

Purpose

AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

LifeAct-jGCaMP8s

Use

AAV

Tags

LifeAct-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s

Plasmid

#200400

Purpose

Expresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider ant

Depositor

Daniel Kronauer

Article

Hart et al Cell. 2023 Jul 6;186(14):3079-3094.e17.

Inserts

Discosoma red fluorescent protein
Q factor 2
GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

Plasmid

#162385

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Use

Tags

6xHis

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

Plasmid

#218866

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8f

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

Q315L

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

Plasmid

#51083

Purpose

Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV Retrograde

Insert

GCaMP6f

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Mutation

Promoter

hEF1a

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

Plasmid

#216533

Purpose

An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

Depositor

Yuki Kambe

Article

Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

Insert

axon-jGCaMP8m

Use

AAV and Cre/Lox

Tags

GAP43

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

Plasmid

#218862

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8m

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112008

Purpose

For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

Plasmid

#51082

Purpose

Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Mutation

Promoter

hEF1a

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#50942

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

Plasmid

#162384

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Use

Tags

6xHis

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163064

Purpose

Entry vector for: GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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