pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
(Plasmid
#218864)
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PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonederived from pJFRC7
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Backbone manufacturerGerald Rubin
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRSET-jGCaMP8s
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Alt namevariant 688
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SpeciesR. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
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Insert Size (bp)1353
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MutationS26M F286Y Q315H
- Promoter hsp70
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Tag
/ Fusion Protein
- 6xHis-T7 gene 10 leader-Xpress epitope-Enterokinase site (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RSET leader improves expression and basal fluorescence compared to #162386
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10 was a gift from GENIE Project (Addgene plasmid # 218864 ; http://n2t.net/addgene:218864 ; RRID:Addgene_218864) -
For your References section:
jGCaMP8 Variants with Improved Expression for Drosophila. Reagents G, Hasseman JP, Turner GC, L Looger L, Bushey D, Reep D, Tsang A, Zheng J, Korff W, Jayaraman V, Kolb I, Zhang Y.. figshare Dataset. 2024. doi: 10.6084/m9.figshare.26231087.v1. 10.6084/m9.figshare.26231087.v1