Addgene

Showing: 21 - 40 of 189 results

Results per page:

Sort results by:

pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-Donor

Plasmid

#60952

Purpose

Porcine ROSA26 locus targeting

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

Porcine ROSA26 homologous ARM

Use

Gene targeting

Tags

Neo EGFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-ZtTA

Plasmid

#26082

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

a loxP-flanked β-geo preceding the tetracycline transactivator tTA

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-G19

Plasmid

#21711

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 G19 (Gt(ROSA)26Sor Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pROSA26-SAT

Plasmid

#208339

Purpose

ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAT sensor

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-SAE

Plasmid

#208338

Purpose

ROSA26 targeting vector for the SAE sensor for ERK1/2 signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAE sensor

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor MCS Rosa26

Plasmid

#37200

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Inserts

Rosa26 left targeting arm
MCS
Rosa26 right targeting arm

Use

Genomic targeting - zinc finger

Tags

Expression

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9(1.1)_NoFlag_hROSA26_Left

Plasmid

#191442

Purpose

Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

Depositor

Yannick Doyon

Insert

hROSA26 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

Plasmid

#191391

Purpose

Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

Depositor

Yannick Doyon

Insert

hCdt1(30-120)

Use

CRISPR and Synthetic Biology

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

Plasmid

#191389

Purpose

Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

Depositor

Yannick Doyon

Insert

hCdt1(1-100)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-mROSA26 ELD

Plasmid

#60026

Purpose

Expression of a TALEN protein specific for mouse ROSA26 locus.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Insert

TALEN-ROSA26

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-mROSA26 KKR

Plasmid

#60025

Purpose

Expression of a TALEN protein specific for mouse ROSA26 locus.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Insert

TALEN-ROSA26

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
STOP-eGFP-ROSA26TV

Plasmid

#11739

Depositor

Klaus Rajewsky

Article

Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

Insert

STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Tags

Expression

Mutation

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
phROSA26-Tet-HA-JSAP1_WT

Plasmid

#208772

Purpose

Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JSAP1 (MAPK8IP3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRosa26-EF1a-hCas9IRESneo

Plasmid

#67987

Purpose

Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-hCas9IRESneopA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT272_(pRosa26-GTET)

Plasmid

#36882

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge