-
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
S7SZ03
Plasmid#163199PurposeInducible expression of UniProt identifier S7SZ03DepositorInsertS7SZ03
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThermoCas9i_ctrl
Plasmid#100982PurposeExpresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA moduleDepositorInsertsCatalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
UseTagsNoneExpressionBacterialMutationchanged aspartate 8 to alanine and histidine 582 …PromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL4369 (pEffector_TnpB2,ωRNA)
Plasmid#210871PurposeExpresses GstTnpB2 and native ωRNA from constitutive promotersDepositorInsertGstTnpB2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512
Plasmid#161875PurposeExpresses Br512 polymerase in E.coliDepositorArticleInsertModified DNA Polymerase I Large Fragment (Br512)
UseTags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialMutationPromoterT7, tetPAAvailable sinceNov. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4672 (pEffector_TnpB3,ωRNA)
Plasmid#210872PurposeExpresses GstTnpB3 and native ωRNA from constitutive promotersDepositorInsertGstTnpB3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4673 (pEffector_TnpB4,ωRNA)
Plasmid#210873PurposeExpresses GstTnpB4 and native ωRNA from constitutive promotersDepositorInsertGstTnpB4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKAR2-Br512_Mut235
Plasmid#179278PurposeExpresses Br512_Mut235 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (HP47)…ExpressionBacterialMutationPromoterT7Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-S308P-H309D-pKK223
Plasmid#131378PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.DepositorInsertEcNGsC MutY chimera S308P H309D
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-S308A-pKK223
Plasmid#131379PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A S308A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are fro…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307Y-pKK223
Plasmid#131381PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.DepositorInsertEcNGsC MutY chimera F307Y
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-F307A-pKK223
Plasmid#131382PurposeLow level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.DepositorInsertEcNGsC MutY chimera F307A
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only