S7SZ03
(Plasmid
#163199)
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PurposeInducible expression of UniProt identifier S7SZ03
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 163199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS7SZ03
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Alt nameflavoprotein oxidoreductase
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SpeciesGeobacillus sp.
- Promoter T7
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- 10xHis (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert name is a UniProt identifier
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S7SZ03 was a gift from Martin Engqvist (Addgene plasmid # 163199 ; http://n2t.net/addgene:163199 ; RRID:Addgene_163199) -
For your References section:
Experimental and computational investigation of enzyme functional annotations uncovers misannotation in the EC 1.1.3.15 enzyme class. Rembeza E, Engqvist MKM. PLoS Comput Biol. 2021 Sep 23;17(9):e1009446. doi: 10.1371/journal.pcbi.1009446. eCollection 2021 Sep. 10.1371/journal.pcbi.1009446 PubMed 34555022