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Showing: 21 - 40 of 72 results

  2. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Use
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

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  3. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  4. pRL1075

    Plasmid
    #70688
    Purpose
    Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain
    Depositor
    Peter Wolk
    Article
    Black et al Mol Microbiol. 1993 Jul;9(1):77-84.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70688-plasmid-map-sequence-id-126455

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  9. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  16. pRL542

    Plasmid
    #70282
    Purpose
    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70282-plasmid-map-sequence-id-125718

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Showing: 21 - 40 of 72 results