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pAluYa5-neo-TET

Plasmid

#51283

Purpose

expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

AluYa5

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

upstream pol III enhancer sequence of the 7SL gen…

Availability

Academic Institutions and Nonprofits only

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L1-neo-TET

Plasmid

#51284

Purpose

expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

L1RE1 (L1RE1 Human)

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p7SL-neo-TET

Plasmid

#51285

Purpose

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

7SL RNA (RN7SL2 Human)

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

7SL upstream pollII enhancer

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA1-CH-mneo

Plasmid

#51288

Purpose

contains the fully codon optimized ORF1 and ORF2 of the human L1RP

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 (L1RE1 Human)

Use

Tags

mneoI indicator cassette containing an inverted n…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBudORF2-CH

Plasmid

#51289

Purpose

expresses the codon optimized ORF2 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 ORF2 (L1RE1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBudORF1-CH

Plasmid

#51290

Purpose

expresses the codon optimized ORF1 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1 ORF1 (L1RE1 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLenti.tie1.H2B.eYFP.W

Plasmid

#51293

Purpose

Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter

Depositor

Rusty Lansford

Article

Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi:

Insert

H2B-eYFP (H2BC21 Human)

Use

Lentiviral

Tags

H2B-eYFP

Expression

Mutation

Promoter

mTie1

Availability

Academic Institutions and Nonprofits only

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pRGEB31

Plasmid

#51295

Purpose

Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

Type

Empty backbone

Use

CRISPR; Plant expression

Tags

Expression

Plant

Mutation

Promoter

Rice snoRNA U3 and dual 35S promoter

Availability

Academic Institutions and Nonprofits only

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YES1 Y537F

Plasmid

#51299

Purpose

Expression of YES1 Y537F

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCIOX

Plasmid

#51300

Purpose

E.coli expression vector for His8-SUMO tagged recombinant protein

Depositor

Andrea Mattevi

Type

Empty backbone

Use

Tags

His8 + SUMO

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-ERG

Plasmid

#51301

Purpose

Expression of ERG after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-HOXA9

Plasmid

#51302

Purpose

Expression of HOXA9 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

homeobox A9 (HOXA9 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

n/a

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-MYB

Plasmid

#51305

Purpose

Expression of MYB after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-myb avian myeloblastosis viral oncogene homolog (MYB Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2

Plasmid

#51309

Purpose

Expresses far-red fluorescent protein mNeptune2 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge