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Addgene

pUC19
(Plasmid #50005)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 50005-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

Backbone

  • Vector backbone
    pUC6, Addgene plasmid #49793
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 2686
  • Vector type
    cloning vector
  • Promoter lac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Identical to pUC18 (Addgene plasmid #50004) except that the multiple cloning sites (MCS) are arranged in opposite orientation.

Information for Cloning Grade DNA (Catalog # 50005-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 was a gift from Joachim Messing (Addgene plasmid # 50005 ; http://n2t.net/addgene:50005 ; RRID:Addgene_50005)
  • For your References section:

    Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Norrander J, Kempe T, Messing J. Gene. 1983 Dec;26(1):101-6. 10.1016/0378-1119(83)90040-9 PubMed 6323249