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pCAS9-mCherry-Frame +0
Plasmid#66939PurposeCRISPaint frame selector +0DepositorInsertgRNA frame +0
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CLIP170-N-18
Plasmid#54044PurposeLocalization: Microtubule Binding , Excitation: 487, Emission: 509DepositorInsertCLIP170 (CLIP1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMTMVi-M
Plasmid#118757PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-p14
Plasmid#42330DepositorInsertp14 (LAMTOR2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR V7
Plasmid#86856PurposeFluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)DepositorInsertAndrogen receptor (AR) (AR Human)
UseTagsEGFPExpressionMammalianMutationAlternative splice variant 7 (alteration/deletion…PromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDA_078
Plasmid#158582PurposeLentiviral expression of BE4max and customizable gRNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationCas9 D10APromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EBNA1BP2_WT_V5
Plasmid#82932PurposeGateway Donor vector containing EBNA1BP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertEBNA1BP2 (EBNA1BP2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mNeonGreen
Plasmid#98875PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535VA
Plasmid#46887PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLYS1-FLAG-MitoGFP-HA
Plasmid#50057PurposeExpression of GFP targeted to mitochondria with Flag and HA tagsDepositorInsertMito-GFP
UseLentiviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-SENP6
Plasmid#42887DepositorInsertSENP6 (SENP6 Human)
UseTags3xFLAGExpressionMammalianMutationPlease see depositor commentsPromoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbB19k-GFP
Plasmid#126135PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+IMS-HyPer7
Plasmid#136469PurposeMammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsSMAC/DIABLOExpressionMammalianMutationPromoterCMV, SP6Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6622 MSCV-P C HA GLTSCR
Plasmid#34902DepositorInsertGLTSCR1 (BICRA Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE-Plus
Plasmid#214079PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
nuclear EKAR (EGFP-mRFP)
Plasmid#18682DepositorInsertERK Activity Reporter
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-mNeongreen
Plasmid#182388PurposeBacterial expression of Vesicle Nucleating peptide6-mNeongreen fusionDepositorInsertVNp6-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn-pOUT
Plasmid#213905PurposeMariner transposon with outward-facing pJ23104 promoterDepositorInsertmariner transposon with kan resistance and outward-facine pJ23104 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag S127A YAP
Plasmid#27370DepositorInsertYAP (YAP1 Human)
UseTagsflagExpressionMammalianMutationS127A ( major phosphorylation site in YAP2)PromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG241 FKBP-CFAST10
Plasmid#130814PurposeExpresses FKBP-CFAST10 in mammalian cellsDepositorInsertFKBP-CFAST10
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFAT luciferase reporter
Plasmid#10959DepositorInsertIL-2 promoter (IL2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-C1
Plasmid#54611PurposeLocalization: C1 Cloning Vector, Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)
Plasmid#163047PurposeGCaMPer calcium sensor (endoplasmic reticulum)DepositorInsertGCaMPer
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF
Plasmid#225828PurposeA component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)DepositorInsertmVenus-FKBP-K7GEF
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSNK2B
Plasmid#23359DepositorInsertCSNK2B (CSNK2B Human)
UseGateway donor vectorTagsExpressionMutationC138TPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3058
Plasmid#144534PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEBF1 (EBF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfCCK-0.5k-Venus-WPRE
Plasmid#156392PurposeExpresses Venus under CCK gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
