pLYS1-FLAG-MitoGFP-HA
(Plasmid #50057)

• Purpose: Expression of GFP targeted to mitochondria with Flag and HA tags
• Depositing Lab: Vamsi Mootha
• Publication: Sancak et al Science. 2013 Nov 14.

Backbone

• Vector backbone: pLYS1
• Backbone size w/o insert (bp): 7331
• Total vector size (bp): 8341
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mito-GFP
• Insert Size (bp): 717
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: custom

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry