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pTarget_AT1R-tTA

Plasmid

#222851

Purpose

Mammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activator

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Use

Tags

FLAG and tTA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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AICSDP-49: MYL2-mEGFP

Plasmid

#114414

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE-4A6-vioC-3A2-vioD

Plasmid

#73440

Purpose

Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC-P3A2-vioD

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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AICSDP-8:TOMM20-mEGFP

Plasmid

#87423

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-46: MYL7-mEGFP

Plasmid

#114413

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AIF 1-90 mCherry FRB

Plasmid

#67530

Purpose

For expression of mCherry fused FRB to the mitochondrial innermembrane

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Inserts

AIF a.a. 1 - 90
mCherry
FRB

Use

Tags

AIF 1 - 90, FRB, and mCherry

Expression

Mammalian

Mutation

T->L

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE_HRASV12_Hyg

Plasmid

#195143

Purpose

HRas G12V overexpression vector with hygroymycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

hRAS (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Tags

Expression

Mutation

Promoter

pmSyn1

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Lb

Plasmid

#138096

Purpose

Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-Lb

Plasmid

#138105

Purpose

Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133-STU-Lb

Plasmid

#138102

Purpose

Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132-STU-Lb

Plasmid

#138099

Purpose

Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Use

Tags

His-tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-pT

Plasmid

#138108

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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AICSDP-13:FBL-mEGFP

Plasmid

#87427

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

FBL Homology Arms with linker-mEGFP (FBL Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AICSDP-10:LMNB1-mEGFP

Plasmid

#87422

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLX209-neo-HA-Ub-T2A-Tet3G

Plasmid

#202436

Purpose

Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

Ubiquitin

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only