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Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs.

Roberts B, Hendershott MC, Arakaki J, Gerbin KA, Malik H, Nelson A, Gehring J, Hookway C, Ludmann SA, Yang R, Haupt A, Grancharova T, Valencia V, Fuqua MA, Tucker A, Rafelski SM, Gunawardane RN
Stem Cell Reports. 2019 May 14;12(5):1145-1158. doi: 10.1016/j.stemcr.2019.03.001. Epub 2019 Apr 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
114411AICSDP-34: TNNI1-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
114412AICSDP-45: TTN-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
114413AICSDP-46: MYL7-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette
114414AICSDP-49: MYL2-mEGFPHomology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

Antibodies from Article