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Plasmid#202663PurposeTet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassetteDepositorInsertMCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
UseTagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREDSIX
Plasmid#74103PurposemCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other AlphaproteobacteriaDepositorTypeEmpty backboneUseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-ctrl guides
Plasmid#168247Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertRacFRET
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3A WT
Plasmid#123069PurposeGateway entry clone encoding human MAP1LC3A wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA
Plasmid#194242PurposeExpresses human alpha-Synuclein A53TDepositorInsertaSynuclein(A53T) (SNCA Human)
UseAAVTagsHAExpressionMutationA53TPromoterSAR-CAGAvailable sinceJan. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6c:sgRNA#4
Plasmid#64248Purposeexpresses sgRNA under U6c promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-iCre-P2A-tdTomato
Plasmid#73351PurposeExpress iCre and tdTomato under CMV promoterDepositorInsertCMV-iCre-tdtomato
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A6
Plasmid#132065PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A6 (SLC30A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-GGB
Plasmid#198233PurposeWheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411DepositorInsertsGRF4-GIF1 chimera cassette
pPLTP::BBM cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterUbiquitin and maize PLTPAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-NES-jGCaMP8m-WPRE
Plasmid#186045PurposeAAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expressionDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sfGFP
Plasmid#125747PurposesfGFP without iCreERT2 fusionDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1-Trim41-PA-1D4
Plasmid#186339PurposeExpress Trim41-PA-1D4 under the mouse Clgn promoter.DepositorInsertTrim41 (Trim41 Mouse)
UseTags1D4 and PAExpressionMammalianMutationPromoterpClgn1.1Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBJ1806 pRS413-COF1RFP
Plasmid#37101DepositorInsertCOF1-RFP
UseTagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …PromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
CTV
Plasmid#27803DepositorInsertCerulean Traf Venus
UseFret standardTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mClover3-CD4-bla
Plasmid#179448PurposeDonor vector to knock in mClover3 N-terminal to human PER2 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only