pClgn1.1-Trim41-PA-1D4
(Plasmid
#186339)
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PurposeExpress Trim41-PA-1D4 under the mouse Clgn promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepClgn1.1
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Backbone manufacturerMasahito Ikawa
- Backbone size w/o insert (bp) 3793
- Total vector size (bp) 5774
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrim41
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SpeciesM. musculus (mouse)
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Insert Size (bp)1989
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Entrez GeneTrim41 (a.k.a. AW552703, BC020156, R75223, RINCK)
- Promoter pClgn1.1
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Tags
/ Fusion Proteins
- PA (C terminal on insert)
- 1D4 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5'-TTGAGCGGGCCGCTTGCGCACTGG-3'
- 3′ sequencing primer 5'-GCCACACCAGCCACCACCTTCTG-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pClgn1.1-Trim41-PA-1D4 was a gift from Masahito Ikawa (Addgene plasmid # 186339 ; http://n2t.net/addgene:186339 ; RRID:Addgene_186339) -
For your References section:
Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, Ikawa M. PLoS Genet. 2022 Jun 1;18(6):e1010241. doi: 10.1371/journal.pgen.1010241. 10.1371/journal.pgen.1010241 PubMed 35648791
