Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 3881 - 3900 of 145459 results
  1. B0030m_BC

    Plasmid
    #74394
    Purpose
    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]
    Depositor
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pFastBacI/MDR3cysless

    Plasmid
    #74400
    Purpose
    expression of MDR3 in insect cells
    Depositor
    Insert
    MDR3 (Abcb1a Mouse)
    Use
    Tags
    10His
    Expression
    Insect
    Mutation
    all Cys to Ala
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
  3. FLAGHis10pFastBacHTA/Serca1

    Plasmid
    #74401
    Purpose
    expression of SERCA in insect cells
    Depositor
    Insert
    SERCA1 (ATP2A1 rabbit)
    Use
    Tags
    10His and FLAG
    Expression
    Insect
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1/HisA/Serca1

    Plasmid
    #74402
    Purpose
    expression of SERCA in mammalian cells
    Depositor
    Insert
    SERCA1 (ATP2A1 rabbit)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  6. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  7. pBABE.is4-mOpn

    Plasmid
    #74410
    Purpose
    Expresses mouse Osteopontin from transcript tv4 in mammalian cells
    Insert
    mouse Opn (Spp1 Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Availability
    Academic Institutions and Nonprofits only
  8. pBABE.is2-mOpn

    Plasmid
    #74411
    Purpose
    Expresses mouse Osteopontin from transcript tv2 in mammalian cells
    Insert
    mouse Opn (Spp1 Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Availability
    Academic Institutions and Nonprofits only
Showing: 3881 - 3900 of 145459 results