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pcDNA-RELΔ424-490

Plasmid

#27267

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Insert

REL (REL Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of transactivation domain I (aa 425-490)

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pBCMV-MS2CP-eDHFR

Plasmid

#167309

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

MS2CP-eDHFR

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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PCD FL1P

Plasmid

#12567

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

One perfect CXCR4 binding site (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Use

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
PCD FL6X

Plasmid

#12566

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

Four CXCR4 imperfect binding sites (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
PCDNA3-TAF4(827-909)

Plasmid

#105614

Purpose

transient express TAF4 HFD in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD amino acid 827-909 (Taf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Use

Tags

Flag and FokI nuclease

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PCD FL2X

Plasmid

#12564

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

Two CXCR4 imperfect binding sites (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA mouse NHERF-1 (1-325)

Plasmid

#32706

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Deleted last 30 AA putative Ezrin binding site

Promoter

CMV

Available since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 1-225 (#431)

Plasmid

#99927

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-225

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PCD FL4X

Plasmid

#12565

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

Four CXCR4 imperfect binding sites (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
dCas9-FCPF

Plasmid

#220131

Purpose

Expresses dCas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 S. pyogenes, Synthetic)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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L2111-FKBP-GPA-20-11S

Plasmid

#222882

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2375-FKBP-GPA-20-11S

Plasmid

#222897

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2376-FKBP-GPA-20-114

Plasmid

#222896

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2374-FRB-GPA-20-11S

Plasmid

#222895

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc11S

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2373-FRB-GPA-20-114

Plasmid

#222894

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2179-FRB-GPA-6-GFPS

Plasmid

#222893

Purpose

Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-6GS-GFPS

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2110-FKBP-GPA-20-114

Plasmid

#222881

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge