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Addgene

pcDNA 3.1 (+) Tac-delta scr
(Plasmid #80019)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80019 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)hygro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tac
  • Alt name
    CD25
  • Species
    H. sapiens (human)
  • Mutation
    CD25 amino acids 1 to 263 fused to KLRFGFK
  • Entrez Gene
    IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
  • Promoter CMV
  • Tag / Fusion Protein
    • KLRFGFK (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1 (+) Tac-delta scr was a gift from Chinten James Lim (Addgene plasmid # 80019 ; http://n2t.net/addgene:80019 ; RRID:Addgene_80019)
  • For your References section:

    The membrane-proximal KXGFFKR motif of alpha-integrin mediates chemoresistance. Liu CC, Leclair P, Yap SQ, Lim CJ. Mol Cell Biol. 2013 Nov;33(21):4334-45. doi: 10.1128/MCB.00580-13. Epub 2013 Sep 3. 10.1128/MCB.00580-13 PubMed 24001772