pcDNA 3.1 (+) Tac-delta scr
(Plasmid
#80019)
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PurposeMammalian expression of Tac-delta scr
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)hygro
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTac
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Alt nameCD25
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SpeciesH. sapiens (human)
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MutationCD25 amino acids 1 to 263 fused to KLRFGFK
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Entrez GeneIL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
- Promoter CMV
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Tag
/ Fusion Protein
- KLRFGFK (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1 (+) Tac-delta scr was a gift from Chinten James Lim (Addgene plasmid # 80019 ; http://n2t.net/addgene:80019 ; RRID:Addgene_80019) -
For your References section:
The membrane-proximal KXGFFKR motif of alpha-integrin mediates chemoresistance. Liu CC, Leclair P, Yap SQ, Lim CJ. Mol Cell Biol. 2013 Nov;33(21):4334-45. doi: 10.1128/MCB.00580-13. Epub 2013 Sep 3. 10.1128/MCB.00580-13 PubMed 24001772