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Showing: 1 - 10 of 10 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  2. Luciferase Plasmids

    Type
    Collection
    ... processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created ...Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87066 pcDNA3.1-ccdB-Firefly Firefly Creation of C-terminal Firefly...fusions using Gateway cloning Mikko Taipale 87068 pcDNA3.1-Renilla-ccdB Renilla Creation of N-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87069 pcDNA3.1-Firefly-ccdB Firefly Creation of N-terminal Firefly...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Tuschl 26397 pF151 pcDNA3.1(+)SOD1WT SOD1 CMV ALS Elizabeth Fisher 26398 pF152 pcDNA3.1(+)SOD1A4V SOD1 CMV...Fisher 26399 pF153 pcDNA3.1(+)SOD1G37R SOD1 CMV ALS Elizabeth Fisher 26400 pF154 pcDNA3.1(+)SOD1G85R SOD1... Xu 29340 pcDNA3.1/GS-DJ1-R98Q-V5 PARK7 His, V5 CMV Parkinson's Mark Cookson 29341 pcDNA3.1/GS-DJ1-E18N-V5...Cookson 29342 pcDNA3.1/GS-DJ1-E18Q-V5 PARK7 V5 CMV Parkinson's Mark Cookson 29343 pcDNA3.1/GS-DJ1-L166P-V5...Cookson 29344 pcDNA3.1/GS-DJ1-E64D-V5 PARK7 His, V5 CMV Parkinson's Mark Cookson 29345 pcDNA3.1/GS-DJ1-D149A-V5...Cookson 29410 pcDNA3.1/GS-DJ1-C53A PARK7 His, V5 CMV Parkinson's Mark Cookson 29411 pcDNA3.1/GS-DJ1-A104T...Parkinson's Mark Cookson 29527 pcDNA3.1-ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ..., pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla...

  7. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells...

  8. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

  10. Cre-lox system

    Type
    Collection
    ...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...
Showing: 1 - 10 of 10 results