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Addgene

PCD FL1P
(Plasmid #12567)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12567 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Luciferase ; Reporter for translation repression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    One perfect CXCR4 binding site
  • Alt name
    CXCR4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    40
  • Entrez Gene
    CXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1)
  • Tag / Fusion Protein
    • luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer BGH rev primer
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. One perfect complementary binding site to the antisense strand of CXCR4 siRNA was cloned in the XbaI/ApaI sites in the 3'UTR of luciferase.

Perfect binding site sequence is - 5'-AAGTTTTCACTCCAGCTAACA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCD FL1P was a gift from Carl Novina (Addgene plasmid # 12567 ; http://n2t.net/addgene:12567 ; RRID:Addgene_12567)
  • For your References section:

    Recapitulation of short RNA-directed translational gene silencing in vitro. Wang B, Love TM, Call ME, Doench JG, Novina CD. Mol Cell. 2006 May 19. 22(4):553-60. 10.1016/j.molcel.2006.03.034 PubMed 16713585