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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH2-FLAG

Plasmid

#66806

Purpose

Tet-inducible expression of IDH2 in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH2 (IDH2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-bTrCP

Plasmid

#20718

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

beta-transducin repeat containing (BTRC) (Btrc Mouse)

Use

Tags

Myc3

Expression

Mammalian

Mutation

*

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-CoV-2-S-RBD-8his

Plasmid

#145145

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)

Use

Tags

8his tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only
pLenti.PGK.LifeAct-Ruby.W

Plasmid

#51009

Purpose

Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

Depositor

Rusty Lansford

Insert

LifeAct-mRFPruby

Use

Lentiviral

Tags

LifeAct

Expression

Mammalian

Mutation

Promoter

human PGK

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pBJ1-human b2m

Plasmid

#12099

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

human beta-2-microglobulin (B2M Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Use

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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mPlum-N1

Plasmid

#54629

Purpose

Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Neo(+) hSUMF1

Plasmid

#24386

Depositor

Carolyn Bertozzi

Article

Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

hSUMF1 (SUMF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCX-OKS-2A

Plasmid

#19771

Purpose

Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

Depositor

Shinya Yamanaka

Article

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Three genes are connected with the foot-and-mouth…

Promoter

Available since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRU1103

Plasmid

#14467

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

lacZ (lacZ E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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GeNL/pcDNA3

Plasmid

#85200

Purpose

Green color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Green enhanced Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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