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Plasmid#119945PurposeExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS pcDNA3
Plasmid#206057Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-BCR-ABL-p190-FLAG
Plasmid#205620PurposeExpression of BCR-ABL oncogene in mammalian cellsDepositorInsertBCR-ABL oncogene, p190 isoform b3a2
UseTagsFLAGExpressionMammalianMutationT117M- Please see depositor commentsPromoterCMVAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-eGFP
Plasmid#104834PurposeeFFly luciferase for invivo Imaging, eGFP for flourescent detectionDepositorInsertseFFly
eGFP
UseLentiviral and Luciferase; EgfpTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
Plasmid#171019PurposeLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cellsDepositorInsertLargeBit-SNAPtag- Beta-actin
UseLentiviral and LuciferaseTagsBeta actin and LargeBiTExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)Luc
Plasmid#200112PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence in minimal p…Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Human, Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFG005_Spike_Inducible_3xFlag
Plasmid#191578PurposeInducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseLentiviralTags3x FLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLuc
Plasmid#83281Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)DepositorInsertFirefly Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE
Plasmid#123363PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.DepositorInsertEGFP-NLS
UseCRISPR, Lentiviral, and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E_inD)Luc
Plasmid#200109PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
UseTagsExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element in front of EGR1 promoter…Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only