Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 3721 - 3740 of 4262 results

  2. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

    Enlarge

  3. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

    Enlarge

  4. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR T757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

    Enlarge

  5. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

    Enlarge

  6. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

    Enlarge

  7. pIS1

    Plasmid
    #12179
    Depositor
    David Bartel
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    12179-plasmid-map-sequence-id-234698

    Enlarge

  8. pLEW82v4

    Plasmid
    #24009
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24009-plasmid-map-sequence-id-11280

    Enlarge

  9. pLEW100v5

    Plasmid
    #24011
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24011-plasmid-map-sequence-id-11282

    Enlarge

  10. pLEW100v5-HYG

    Plasmid
    #24012
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24012-plasmid-map-sequence-id-11283

    Enlarge

  11. pSFG-tcLuc+ITEA-

    Plasmid
    #22250
    Depositor
    Ananda Roy
    Insert
    Luciferase
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. pLEW100v5-177-HYG

    Plasmid
    #24013
    Depositor
    George Cross
    Insert
    Luciferase
    Use
    Trypanosome expression
    Tags
    Expression
    Mutation
    K520R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    24013-plasmid-map-sequence-id-392337

    Enlarge

  13. Luc.Cre empty vector

    Plasmid
    #20905
    Depositor
    Tyler Jacks
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    20905-plasmid-map-sequence-id-339882

    Enlarge

  15. pMal-HA

    Plasmid
    #209346
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209346-plasmid-map-sequence-id-419547

    Enlarge

  16. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha and EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

    Enlarge

  17. pMal-Strep

    Plasmid
    #209345
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209345-plasmid-map-sequence-id-419544

    Enlarge

  18. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

    Enlarge

  19. pMal-Halo

    Plasmid
    #209349
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209349-plasmid-map-sequence-id-419545

    Enlarge

  20. pSmart-Nm-sgRNA-BbsI

    Plasmid
    #49157
    Purpose
    Plasmid for cloning spacer into sgRNA for NmCas9
    Depositor
    James Thomson
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    49157-plasmid-map-sequence-id-72446

    Enlarge

Showing: 3721 - 3740 of 4262 results