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Showing: 7401 - 7440 of 148587 results

  2. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid
    #207404
    Purpose
    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]
    Depositor
    Alexandros Poulopoulos
    Article
    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10
    Insert
    Cas9-RC
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP-2A
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Nov. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    207404-plasmid-map-sequence-id-413204

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  3. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid
    #128606
    Purpose
    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128606-plasmid-map-sequence-id-254156

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  4. qTAG-N-Puro-dTAG

    Plasmid
    #207694
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207694-plasmid-map-sequence-id-413807

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  5. EBFP-C1

    Plasmid
    #54738
    Purpose
    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54738-plasmid-map-sequence-id-256475

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  7. pCMVMagFRET-1

    Plasmid
    #50742
    Purpose
    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Insert
    gene encoding for MagFRET-1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50742-plasmid-map-sequence-id-327713

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  8. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    pMecp2
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  9. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid
    #200410
    Purpose
    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor detecting iRFP720
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200410-plasmid-map-sequence-id-396811

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  11. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  14. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  18. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

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  22. pLenti-Tet

    Plasmid
    #138086
    Purpose
    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles
    Depositor
    Eva Frickel
    Article
    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    minimal CMV promoter with heptamerized tet‐operat…
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138086-plasmid-map-sequence-id-276295

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  29. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

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  38. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Tags
    Expression
    Worm
    Mutation
    Promoter
    T7
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

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  39. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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Showing: 7401 - 7440 of 148587 results