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PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSC-SP-PW-GFP
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Backbone manufacturerInder Verma Lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2 and mCherry
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SpeciesSynthetic
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Insert Size (bp)1415
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-CAG-FLEX-BFP2-(mCherry)’ was a gift from Ian Wickersham (Addgene plasmid # 115234 ; http://n2t.net/addgene:115234 ; RRID:Addgene_115234) -
For your References section:
"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification. Jin L, Matsuyama M, Sullivan HA, Zhu M, Lavin TK, Hou Y, Lea NE, Pruner MT, Dam Ferdinez ML, Wickersham IR. Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2023481120. doi: 10.1073/pnas.2023481120. Epub 2023 Feb 6. 10.1073/pnas.2023481120 PubMed 37053554