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Plasmid#84306PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.DepositorInsertBarx1 (Barx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-FlagmuEzh2deltaset-Hygro
Plasmid#49403PurposeExpresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.DepositorInsert3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
UseRetroviralTagsFlagExpressionMammalianMutationSet domain deletedPromoterMSCV promotorAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-His6
Plasmid#51536PurposeExpress His6-tagged mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1
Plasmid#99505Purpose3rd generation lentiviral expression of wild-type Becn1DepositorInsertBeclin 1 (Becn1 Mouse)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBetaActin-FKBP-mScarlet-Dync1h1motor
Plasmid#191330PurposeExpresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
UseTagsExpressionMammalianMutationDYNC1H1 motor domain aa 1453-4644PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMSV-Myf5 (puro)
Plasmid#14711DepositorInsertMyf5 (Myf5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shNCLX-2
Plasmid#181871PurposeExpresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertsolute carrier 8 family member B1 (Slc8b1 Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shNCLX-1
Plasmid#181870PurposeExpresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertsolute carrier 8 family member B1 (Slc8b1 Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EGFP.T2A.NCLX
Plasmid#181873PurposeExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoterDepositorInsertsUseAAV and AdenoviralTagsHA and mycExpressionMammalianMutationPromotersynthetic hybrid CAG promoter and synthetic hybri…Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR26-mBMP7
Plasmid#127374PurposeAllows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MCHR1-N-10
Plasmid#55078PurposeLocalization: Cilium, Excitation: 587, Emission: 610DepositorInsertMCHR1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-CCSS
Plasmid#83424PurposeMouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidues 218 and 219 from C to S (CCSS: TGCTGT -&…PromoterCMVAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE
Plasmid#127868PurposepAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoterDepositorInsertNos1 (Nos1 Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationAmino acids 1-133 onlyPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag PGC1 T177A S538A
Plasmid#18093DepositorInsertPGC-1a (Ppargc1a Mouse)
UseTagsFlagExpressionMammalianMutationT177A S538APromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
cytosolic moPrP (3F4)
Plasmid#1321DepositorInsertPrP (Prnp Mouse)
UseTagsExpressionMammalianMutationN-term truncation (to make gene cytosolic), vario…PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T562A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T562D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXJ40-HA-Merlin I
Plasmid#19699DepositorInsertMerlin I (Nf2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Rab10-FLAG
Plasmid#135559PurposeExpress mouse Rab10 in mammalian cells, FLAG at N terminalDepositorInsertRab10 (Rab10 Mouse)
UseTagsFlagExpressionInsectMutationPromoterT7 promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only