pAAV-shNCLX-1
(Plasmid #181870)

• Purpose: Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
• Depositing Lab: Hilmar Bading
• Publication: Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10.1016/j.jbc.2021.101508.

Backbone

• Vector backbone: pAAV-U6_CaMKIIa-mCherry
• Vector type: Mammalian Expression, Mouse Targeting, Adenoviral, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: solute carrier 8 family member B1
• Alt name: NCLX
• Alt name: sodium/calcium/lithium exchanger
• gRNA/shRNA sequence: ATGTTGGACTGTGGATCTAAA
• Species: M. musculus (mouse)
• GenBank ID: NM_133221
• Entrez Gene: Slc8b1 (a.k.a. AF261233, NCKX6, NCLX, Slc24a6)
• Promoter: U6
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamhI (not destroyed)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: TTGGGTAGTTTGCAGTTTTAAAATT
• 3′ sequencing primer: TCACTGAGGAAGCTCTCTG

Resource Information

• Supplemental Documents:
  • 041_U6-sh(MsNCLX-1)_CKIIa-mCherry.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry