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CN2251-rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#164453

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1002-pAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163486

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP1002 - pAAV-AiE2016m-minBG-EGFP-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163475

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CN2249-rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#164452

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA

Plasmid

#20296

Purpose

Cre-activated AAV expression of EYFP

Depositor

Karl Deisseroth

Insert

EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-EGFP

Plasmid

#51093

Purpose

Forebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled EGFP fluorophore

Depositor

Jonathan Ting

Insert

oChIEF(E163A/T199C)

Use

AAV

Tags

P2A-EGFP

Expression

Mammalian

Mutation

E163A/T199C

Promoter

CaMKIIa

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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CN2255-rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#164455

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Tags

Expression

Mutation

Promoter

CamKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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CN1203-scAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#163492

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBetaGlobin

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

ChroME-ST

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

Inserts

mNeonGreen
eKaz luciferase
Volvox Channelrhodopsin 1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Expression

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1078-pAAV-mscRE16-minBGpromoter-oNigri-WPRE-hGHpA

Plasmid

#163490

Purpose

Direct-expressing oNigri AAV Virus. Alias: AiP1078 - pAAV-AiE2016m-minBG-oNigri-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

oNigri

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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AiP1918 - pAAV-AiE2454m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214578

Purpose

AiE2454m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1776 - pAAV-AiE2467m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214542

Purpose

AiE2467m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163485

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP995 - pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1892 - pAAV-AiE2579m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214572

Purpose

AiE2579m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1731 - pAAV-AiE2386m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214531

Purpose

AiE2386m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge