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Precise multimodal optical control of neural ensemble activity.

Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov K, Brohawn SG, Waller L, Adesnik H
Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 10.1038/s41593-018-0139-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108902pCAG-ChroME-mRuby2-STCation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation
108912pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
108960pCAG-H2B-mRuby3-IRES-eGtACR1-STAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence
109048pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-STAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
109124pCAG-C1V1-TT-mRuby2-STCation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109125pCAG-ChR2-mRuby2-STCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109126pCAG-ChETA-mRuby2-STCation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109127pCAG-ChroME(F243Y)-mRuby2-STModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109128pCAG-ChroME(S273A)-mRuby2-STModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109129pCAG-Chronos(M140T)-mRuby2-STModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109130pCAG-CsChR-mRuby2-STCation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109131pCAG-CoChR-mRuby2-STCation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
109132pCAG-eiC++-mRuby2-STArtificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites
109133pCAG-ePsuACR-mRuby2-STAnion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites
109134pCAG-eNpHR3.0-mRuby2-STHalorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites
109135pCAG-eArch3.0-mRuby2-STArchaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites
109136pCAG-eGtACR1-mRuby2-STAnion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

Antibodies from Article