Addgene

We narrowed to 4,470 results for: pcr

Showing: 341 - 360 of 4470 results

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pCR4-24XMS2SL-stable

Plasmid

#31865

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

24xMS2 Stem Loops - Stable

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagGFP2-PuroR

Plasmid

#80970

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagGFP2

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Pcr4-12xMBS-PBS

Plasmid

#52984

Purpose

provide MBS-PBS alternating sequence

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

12xMBS-PBS

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPR-RFP entry

Plasmid

#192278

Purpose

Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP flanked by type V-M CRISPR repeat sequences

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA M6

Plasmid

#221229

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mGM-CSF

Plasmid

#74465

Purpose

expression of mGM-CSF in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagRFP-PuroR

Plasmid

#80971

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagRFP

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

ermE*/tipA

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-HaloTag-PuroR

Plasmid

#80960

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

HaloTag

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKalpha

Plasmid

#15467

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-RhoA

Plasmid

#42958

Purpose

Expresses optogenetically controlled RhoA

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCry2PHR-mCh-LRP6c

Plasmid

#42960

Purpose

Expresses optogenetically controlled LRP6 (C-terminal domain)

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Tags

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-GFP

Plasmid

#130277

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-GFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagBFP-PuroR

Plasmid

#80969

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagBFP

Use

Gene tagging

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-RFP

Plasmid

#130276

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-RFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKbeta

Plasmid

#15465

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ELK4

Plasmid

#98617

Purpose

Gateway entry TOPO TA cloning vector for ELK4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Mutation

Promoter

U6; CMV

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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