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Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RN3P-KDM5B-FLAG
Plasmid#86398PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expressionDepositorInsertKDM5B (Kdm5b Mouse)
UseTags3x-FLAGExpressionMammalianMutationPromoterLacAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-hIKAROS-IRES-GFP
Plasmid#74046Purposeretroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1DepositorInserthuman Ikaros (IKZF1) (IKZF1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-ZEB2-P2A-mCherry
Plasmid#203857PurposeTet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertZEB2 (ZEB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_314-HRI-V5
Plasmid#202434PurposeExpression of HRIDepositorInsertHRI (EIF2AK1 Human)
UseCRISPR and LentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-WT-KRAS-IRES-Blast
Plasmid#153335PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cellsDepositorInsertmCherry-WT-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-hPGK-Blast-2A-STING-TurboID
Plasmid#204713PurposeExpresses C-terminal TurboID tagged human STING for proximity ligation.DepositorInsertSTING1 (STING1 Human)
UseLentiviralTagsTurboIDExpressionMammalianMutationPromoterhPGKAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_016-sgATF4-1
Plasmid#202455PurposeExpression of a guide RNA targeting ATF4DepositorInsertATF4 gRNA (ATF4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-HRI-V5-IRES-BFP
Plasmid#202435PurposeLentiviral vector expression of HRI and TagBFPDepositorInsertHRI (EIF2AK1 Human)
UseCRISPR and LentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 D614G
Plasmid#164437PurposeEncodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus productionDepositorInsertSARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-hHelios-IRES-GFP
Plasmid#74047Purposeretroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1DepositorInserthuman Helios (IKZF2) (IKZF2 )
UseRetroviralTagsFLAGExpressionMammalianMutationencodes shorter version of human HeliosPromoterpMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iSeroSnFR
Plasmid#128483PurposeFluorescent reporter for serotonin (bacterial expression)DepositorInsertiSeroSnFR
UseTagsHis, T7, and cpsfGFPExpressionBacterialMutationPromoterT7Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75240PurposeCRISPR/Cas9 plasmid against human IkarosDepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75241PurposeCRISPR/Cas9 plasmid against human IkarosDepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-GemininΔDbox-HA-Puro
Plasmid#193770PurposeDoxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertGemininΔDbox-HA (GMNN Human)
UseLentiviralTagsHAExpressionMutationDbox removed (R23A, L26A). Codons switched at siR…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJHG003
Plasmid#126454PurposeExpresses Cas9-XTEN-dTdT in mammalian cells.DepositorInsertCas9-XTEN-dTdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationAdded D343E and D345E to inactivate TdT.PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-NR4A3-P2A-mCherry
Plasmid#203863PurposeTet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertNR4A3 (NR4A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-NR4A1-P2A-mCherry
Plasmid#203859PurposeTet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertNR4A1 (NR4A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only