-
PurposeFLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 86398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneRN3P
-
Backbone manufacturerDerivative of pBluescript RN3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKDM5B
-
SpeciesM. musculus (mouse)
-
Entrez GeneKdm5b (a.k.a. 2010009J12Rik, 2210016I17Rik, AW556288, D1Ertd202, D1Ertd202e, Jari, Jarid1b, PLU, PLU-1, PUT1, Pl, Plu1, RBBP2H1A, Rb-B, Rb-Bp2, mKIAA4034)
- Promoter Lac
-
Tag
/ Fusion Protein
- 3x-FLAG (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer XBG-R (GACTCCATTCGGGTGTTC) (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RN3P-KDM5B-FLAG was a gift from Wei Xie (Addgene plasmid # 86398 ; http://n2t.net/addgene:86398 ; RRID:Addgene_86398) -
For your References section:
Allelic reprogramming of the histone modification H3K4me3 in early mammalian development. Zhang B, Zheng H, Huang B, Li W, Xiang Y, Peng X, Ming J, Wu X, Zhang Y, Xu Q, Liu W, Kou X, Zhao Y, He W, Li C, Chen B, Li Y, Wang Q, Ma J, Yin Q, Kee K, Meng A, Gao S, Xu F, Na J, Xie W. Nature. 2016 Sep 14;537(7621):553-557. doi: 10.1038/nature19361. 10.1038/nature19361 PubMed 27626382