Addgene

We narrowed to 14,520 results for: LEA

Showing: 3441 - 3460 of 14520 results

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMT3928

Plasmid

#113331

Purpose

control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1100

Plasmid

#110117

Purpose

Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_900

Plasmid

#110116

Purpose

Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_700

Plasmid

#110115

Purpose

Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_500

Plasmid

#110114

Purpose

Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA1

Plasmid

#107076

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA3

Plasmid

#107078

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA3

Plasmid

#107075

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA2

Plasmid

#107077

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA2

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA2

Plasmid

#107074

Purpose

M. sedula PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA1

Plasmid

#107073

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T-MsePCNA2

Plasmid

#107064

Purpose

Expresses M. sedula PCNA2 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T-MsePCNA1

Plasmid

#107063

Purpose

Expresses M. sedula PCNA1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-S3C

Plasmid

#107090

Purpose

S. solfataricus PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge