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Plasmid#64845PurposeA lentiviral vector for the expression of mouse Myt1 under doxycyline controlDepositorInsertMyt1 (Myt1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous T/brachyuryAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNXgate33-3HA
Plasmid#105075PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneUseTags3xHA and NubGExpressionYeastMutationPromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK599s
Plasmid#110601PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-mWasabi-G4
Plasmid#164168PurposeExpresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminalDepositorInsertmWasabi
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation1319C>T; Pro440LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-E25A
Plasmid#82345PurposeExpresses FLAG-tagged SLC30A10-E25A in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationE25APromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Jag2ICD-HA pBacPAK8-3
Plasmid#17332DepositorInsertJagged2 (Jag2 Rat)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBTK301
Plasmid#110593PurposeBTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)DepositorInsertBba_B0015 Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
11.5kb-GFP (pBSK)
Plasmid#29459DepositorInsertSHIP-1 promoter (Inpp5d Mouse)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVenus_Lys-less-Cdc20 (1620)
Plasmid#39849DepositorInsertCdc20 (CDC20 Human)
UseTagsVenusExpressionMammalianMutationLys-less = all Lys converted to ArgPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d10-364delta263-295-TEV-STREP
Plasmid#171830PurposeMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutation10-364delta263-295PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertMIS12-BUB1 (MIS12 Human)
UseTagsEYFPExpressionMammalianMutationBUB1 fused to MIS12PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-ARF4a
Plasmid#67497PurposeExpression of shRNA targeting human ARF4aDepositorInsertHsARF4 shRNA (ARF4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
prNFL-L269P-EGFP
Plasmid#132601PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation806T>C, 807G>C; Leu269ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-P441L-EGFP
Plasmid#132604PurposeExpresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutationDepositorInsertNefl (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation1322C>T, 1323A>G; Pro441LeuPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpLacI:mini35ss (GB0534)
Plasmid#68179PurposeProvides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid partDepositorInsert6xOpLacI:mini35S
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-N43H
Plasmid#82344PurposeExpresses FLAG-tagged SLC30A10-N43H in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationN43HPromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B L69E
Plasmid#175026PurposeExpresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (L69E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationLeucine 69 to Glutamate (CTA to GAA)PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-D248A
Plasmid#82346PurposeExpresses FLAG-tagged SLC30A10-D248A in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationD248APromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)
Plasmid#87202PurposeTo express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationResidues from 90-107 are inserted.PromoterCMVAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD
Plasmid#168161PurposeInducible expression of PmcCAST TnsAB and TnsC proteins.DepositorInsertPmcCAST Tns proteins (TnsAB and TnsC)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS26: pTns(PmcCAST)_ΔTniQ
Plasmid#168159PurposeInducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.DepositorInsertPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS27: pTns(PmcCAST)_ΔTnsD
Plasmid#168160PurposeInducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS25: pTns(PmcCAST)
Plasmid#168158PurposeInducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.DepositorInsertsPmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Jag1ICD-HA pBacPAK8-3
Plasmid#17331DepositorInsertJagged1 (Jag1 Rat)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
UseTagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
RP78 pUAST Orco mRNA
Plasmid#53431PurposeExpresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS controlDepositorInsertOrco (Orco )
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-HA-mIFITM3-K83,88,104A
Plasmid#58405PurposeExpresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysines 83, 88, and 104 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83A
Plasmid#58401PurposeExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 83 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24A
Plasmid#58400PurposeExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 24 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Venus-Cdc20 R132A (1768)
Plasmid#26059DepositorInsertCdc20 (CDC20 Human)
UseTagsVenusExpressionMammalianMutationMutation in the Mad2 binding site, R132APromoterAvailable sinceAug. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-G19
Plasmid#21711DepositorInsertROSA26 G19 (Gt(ROSA)26Sor Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO MycLAP-STIL ∆CC
Plasmid#80268Purposemammalian expression plasmid for MycLAP-STIL coiled-coil deletionDepositorInsertSTIL (STIL Human)
UseTagsMyc-GFPExpressionMammalianMutationmissing conserved coiled-coil (amino acids 721-74…PromoterCMVAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK157_mCherry-4.1G-CTD
Plasmid#46359DepositorInsert4.1G C-terminal domain (EPB41L2 Human)
UseTagsmCherryExpressionMammalianMutationC-terminal domain from 886-1005 amino acidsPromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pChlr-4SYR
Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
C513-E07: attB5r-<p10p-polyhedrinp>-attB1r
Plasmid#162949PurposeGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertdual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-KRasCT
Plasmid#175834PurposeExpress Venus with plasma membrane-targeting sequence in mammalian cellsDepositorInsertVenus with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD_OsU3 (GB1184)
Plasmid#68209PurposeProvides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertOsU3 promoter
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only