11.5kb-GFP (pBSK)
(Plasmid
#29459)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHIP-1 promoter
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Alt nameSHIP1
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SpeciesM. musculus (mouse)
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Insert Size (bp)11500
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Entrez GeneInpp5d (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, p150Ship, s-SHIP)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See attached map ("View Map") and Materials and Methods for complete cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
11.5kb-GFP (pBSK) was a gift from Larry Rohrschneider (Addgene plasmid # 29459 ; http://n2t.net/addgene:29459 ; RRID:Addgene_29459) -
For your References section:
The intron 5/6 promoter region of the ship1 gene regulates expression in stem/progenitor cells of the mouse embryo. Rohrschneider LR, Custodio JM, Anderson TA, Miller CP, Gu H. Dev Biol. 2005 Jul 15. 283(2):503-21. 10.1016/j.ydbio.2005.04.032 PubMed 15978570
Map uploaded by the depositor.