pChlr-4SYR
(Plasmid
#52158)
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PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepChlr-pNEB193
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 2280
- Total vector size (bp) 2344
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Modifications to backboneReplaced the original ampicillin resistance gene Bla with the chloramphenicol resistance gene Cam from pACYC (New England Biolabs) using the Gibson Assembly method.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePumilio homology domain amino acids 133-168
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Alt namePUM-HD module 4
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SpeciesH. sapiens (human)
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Insert Size (bp)36
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MutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in Module 4
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GenBank IDD43951
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Entrez GenePUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pChlr-4SYR was a gift from Huimin Zhao (Addgene plasmid # 52158 ; http://n2t.net/addgene:52158 ; RRID:Addgene_52158) -
For your References section:
Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042